Rapid determination of pigments in tobacco with near infrared spectroscopy

In order to meet the needs of rapid analysis of pigments in tobacco,the near infrared(NIR) spectra of 77 representative tobacco samples were measured with FT-NIR spectroscopy.Based on the spectral data and determined chemical results of tobacco samples,the predictive models of lutein,β-carotene and other carotenoids in tobacco were established by partial least square(PLS) method.The correlation coefficients(R) of the models were 0.9802,0.9962 and 0.9751 respectively,and the root mean square errors of prediction(RMSEP) were 0.00947,0.0607 and 0.0446 respectively.This method was simple,rapid and nondestructive to sample,so it is suitable for rapid determination of lutein,β-carotene and other carotenoids in tobacco samples.