Development of a flow microsensor for selective detection of nitric oxide in the presence of hydrogen peroxide

Abstract The detection of reactive oxygen and nitrogen species is of utmost importance in several pathological situations. Indeed, these reactive species are biomarkers of oxidative stress and their real-time monitoring is crucial to adapt medical treatments. We report here on the electrochemical detection of nitric oxide (NO ) in the presence of hydrogen peroxide (H 2 O 2 ). The detection was performed by using different sensing microdevices involving either static solutions in wells or flowing solutions in microfluidic channels. Furthermore, an original strategy was proposed to further enhance the selectivity of NO detection at Pt/poly(eugenol) modified platinum electrodes by designing a dual-electrode microfluidic device based on a pre-electrolysis of interfering species including H 2 O 2 at an upstream electrode prior to NO detection.