Reliability of morphological and molecular characterization of lightsprouts for differentiation of potato accessions
2016
The study of reliability of morphological characterization of lightsprouts
for differentiation of potato varieties was performed at the Agricultural
Institute of Slovenia in cooperation with Biotechnical Faculty Podgorica in
order to introduce simple method for further characterization of potato
accessions in Montenegrin gene bank. Seven selected, potentially different,
potato accessions preserved in the Montenegrin gene bank were used for
morphological characterization of lightsprouts. Using UPOV guidelines 11
lightsprout traits were estimated. Molecular assessment was carried out in
parallel with morphological characterization by six microsatellite (SSR)
markers. The latter successfully distinguished all accessions but two, while
four different lightsprout phenotypes were identified in morphological
characterization. Though molecular markers showed more strength in resolving
relationships between genotypes, characterization of lightsprouts still
demonstrated its usefulness due to cheap, simple and rapid procedure.
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