Additional file 4: of Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN

Figure S4. PTEN mediates SCD1-induced migration and invasion of SW116 cells. (A) Representative Western blot of SCD1, β-Catenin, STAT3, S6K and JNK in CRC cells transfected with shSCD1 or SCD1 cDNA. (B) Representative Western blot and quantification data of PTEN in SW116 cells transfected with siRNAs for PTEN (si1 and si2). (C) Representative photographs of transwell assays of shSCD1 or shNC-transfected SW116 after being transfected with PTEN siRNAs (siPTEN) or negative control scramble siRNAs (siNC). The scale bar is 100 μm. (D, E) Histograms show the numbers of migrated (D) and invasive (E) SW116 cells. (F) Representative Western blots and quantified results of SCD1, PTEN, Akt, p-Akt (Ser473), p-Akt (Thr308), E-cadherin and vimentin. (TIFF 2175 kb)