The novel Mas agonist, CGEN 856S, promotes Akt/eNOS activation and anti-proliferation with FOXO1 involvement (1097.3)

Biotechnology advances allowed the discovery of a potential Mas agonist, CGEN 856S, which produces several effects resembling those produced by Angiotensin-(1-7). This study evaluated the effect of CGEN 856S on FOXO1 and Akt/eNOS activation, using different cell types. HAEC (Human Aortic Endothelial Cell), A549 and DU145 (human tumoral linages from lungs and prostate, respectively) cell were treated with CGEN 856S with or without previous treatment with A779. The cells were used for immunolocalization of FOXO1, analyzed by confocal microscopy and the nuclear fluorescence intensity was quantified. Mas-transfected CHO (chinese hamster ovary) cells were stimulated with CGEN 856S. Untransfected CHO cells were used as control and the proteins were used for phospho-Akt, phospho-eNOS, phosphor-FOXO1, Mas and GAPDH detection by western blot. A549 and DU145 cells were also used for cytotoxicity test, being treated with CGEN856S with a combination of PI3 kinase inhibitors, Wortmannin or LY294002, in different conce...