Easy washing of lysed cell plugs for bacterial typing by pulsed-field gel electrophoresis using simple equipment

Abstract We designed and tested equipment to wash plugs following cell lysis in pulsed-field gel electrophoresis (PFGE). Our system can wash 30 plugs simultaneously in 1 h using 15 L of Tris–EDTA buffer, which makes plug washing for PFGE less labor-intensive.