e0085 Relationship between the polymorphism of apolipoprotein apo B gene XbaI EcoRI and the serum lipids in the Li nationality of Haina

Objective To study the apolipoprotein (apo) B gene XbaI and EcoRI polymorphism of the Li nationality in Hainan Island and to evaluate their effects on serum apolipoproteins and lipids. Methods The study was carried out in a natural population of 351 (151 samples from the Li nationality and 200 samples from the Han nationality) individuals aged between 20 and 84 from Li nationality in Hainan area. The XbaI and EcoRI polymorphisms of apolipoprotein (apo) B gene were analysed using PCR and Restriction Fragment Length Polymorphism (RFLP) methods. The levels of serum apoA, apoB, total cholesterol (TC), triglyceride (TG), HDL cholesterol (HDL-C) were also measured, and LDL cholesterol (LDL-C) was calculated. Results The frequency of the Li nationality9s X + /X − genotype subgroups was 0.119. The frequency of the Li nationality9s X + allele was 0.059, which was higher than the Han nationality control group (p − /E − genotype subgroups was 0.086. The frequency of the Li nationality9s E − allele was 0.043, which was no difference compared to the Han nationality (p + /X − genotype subgroups were higher than the same group of X − /X − genotype subgroups (p − /X − genotype subgroups (p − /E − genotype subgroups and the E + /E − genotype subgroups (p Conclusions The XbaI genotypes and alleles9 frequency distribution between Li nationality and Han nationality have significant differences but the EcoRI genotypes and alleles9 frequency distribution between Li nationality and Han nationality have no statistical significance (p>0.05). Different genotypes of EcoRI and XbaI have different effects on serum apolipoproteins and lipids. The levels of LDL-C of the genotype subgroups which contains X + allele was higher than the genotype subgroups which does not contain the X + allele. The X + allele can affect lipid levels, but the E − allele can not affect lipid levels.