Relação entre anticorpos Anti-PGL-1 e presença de DNA De Mycobacterium leprae na mucosa nasal de pacientes com hanseníase e contatos

A transmissao da hanseniase e mais intensa em areas hiperendemicas nas quais o numero de individuos clinicamente sadios infectados com Mycobacterium leprae e elevado e pode ser identificado por meio da deteccao da presenca do DNA bacteriano na mucosa nasale teste sorologico com pesquisa de anticorpos IgM contra PGL-I especifico do M.leprae . O objetivo deste estudo foi avaliar a relacao entre presenca de anticorpos IgM anti-PGL-I e a presenca DNA do M.leprae na mucosa nasal em individuos em area endemica. Foram selecionados 337 individuos classificados em 6 grupos: a) 71 contatos consanguineos intradomiciliares – CCOSI, b) 50 contatos consanguineos extradomiciliares – CCOSE, c) 23 contatos nao consanguineos intradomiciliares – CNCOSI, d) 170 contatos nao consanguineos extradomiciliares – CNCOSE, e) 19 pacientes com hanseniase multibacilar – MB e f) 20 pacientes com hanseniase paucibacilar – PB. Dos 71 CCOSI, 19 foram positivos para PCR (26.76%-19/71) e 34 positivos para PGL-I (47.88% - 34/71); de 50 CCOSE, 7 foram positivos para PCR (14.00 - 7/50) e 11 positivos para PGL-I (22.00%, 11/50); de 23 CNCOSI,  4 positivos para PCR (17.39%- 4/23) e 3 positivos para PGL-I (13.04%-3/23); de 170 CNCOSE, 28 positivos para PCR (16.47% - 28/170) e 35 positivos para PGL-I (20.58% -35/170); de 19 pacientes MB, 6 positivos para PCR (31.57%- 6/19) e 11 positivos para PGL-I (57.89% - 11/19) e de 20 pacientes PB, 3 positivos para PCR (15.00% - 3/20) e 4 positivos para PGL-I (20.00%- 4/20). A PCR de swab nasal foi inversamente proporcional ao PGL-I ( p = 0.02, ᵪ² = 5.27), embora ambos os metodos sejam importantes para a vigilância epidemiologica, eles nao possuem correlacao positiva. ABSTRACT Relationship between Anti-PGL-1 antibodies and presence of DNA and Mycobacterium leprae in the nasal mucosa of leprosy patients and contacts. The transmission of leprosy is more intense in hyperendemic areas. The number of clinically healthy individuals infected with Mycobacterium leprae is high. It can be identified by detecting bacterial DNA in the nasal mucosa and serological testing with IgM antibodies against M. leprae specific PGL-I. This study aimed to evaluate the relationship between anti-PGL-I IgM antibodies and the presence of M. leprae DNA in the nasal mucosa in individuals in an endemic area. A total of 337 individuals were selected, classified into 6 groups: a) 71 intradomiciliary consanguineous contacts - CCOSI, b) 50 extradomiciliary consanguineous contacts - CCOSI, c) 23 intradomiciliary non-consanguineous contacts - CNCOSI, d) 170 extradomiciliary non-consanguineous contacts - CNCOSE e) 19 patients with multibacillary leprosy - MB and f) 20 patients with paucibacillary leprosy - PB. Of the 71 CCOSI, 19 were PCR positive (26.76% -19 / 71) and 34 PGL-I positive (47.88% - 34/71); of 50 CCOSE, 7 were PCR positive (14.00 - 7/50) and 11 PGL-I positive (22.00%, 11/50); 23 CNCOSI, 4 PCR positive (17.39% - 4/23) and 3 PGL-I positive (13.04% -3 / 23); 170 CNCOSE, 28 PCR positive (16.47% - 28/170) and 35 PGL-I positive (20.58% -35/170); of 19 MB patients, 6 PCR positive (31.57% - 6/19) and 11 PGL-I positive (57.89% - 11/19) and 20 PB patients, 3 PCR positive (15.00% - 3/20) and 4 positive for PGL-I (20.00% - 4/20). Nasal swab PCR was inversely proportional to PGL-I (p = 0.02, ᵪ² = 5.27). Although both methods are important for epidemiological surveillance, they have no positive correlation.