Abstract 4192: Genome-wide microRNA and functional genomics analysis during differentiation of acute promyelocytic leukemia to granulocytes.

MicroRNA (miRNAs) are short noncoding RNAs that play important roles in human diseases, including cancer, and function to regulate gene expression of critical processes, such as apoptosis, cell proliferation and differentiation, by either translational inhibition or mRNA degradation. However, little is known about the role of miRNAs in granulopoiesis and acute myeloid leukemia (AML). We performed genome-wide miRNAs and functional genomics analysis in the acute promyelocytic leukemia (APL) human cell line HL-60 during its differentiation towards granulocytes, by using miRNA microarrays Exiqon platform, quantitative real time polymerase chain reaction (qRT-PCR) and Ingenuity Pathways Analysis(IPA). Among 1279 human miRNAs analyzed, we identified a unique signature of 86 differentially expressed miRNAs between control and DMSO-treated cells (at p Citation Format: El Habib Dakir, Jun-ichi Satoh, Faustino Mollinedo. Genome-wide microRNA and functional genomics analysis during differentiation of acute promyelocytic leukemia to granulocytes. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4192. doi:10.1158/1538-7445.AM2013-4192