Broadband coherent anti-Stokes Raman scattering (CARS): a potential tool for atherosclerosis diagnostic imaging
2007
Nonlinear optical imaging technologies offer some intriguing medical diagnostic applications. Examples include fast
imaging of elastin and collagen distributions in diseased tissues using two-photon fluorescence (TPF) and second
harmonic generation (SHG), respectively. The 3D sectioning capabilities and biochemical specificity that enable fast
imaging in highly scattering biological media lie at the heart of the appeal of these nonlinear approaches for medical
applications. One of these promising nonlinear techniques relies on the resonance enhancement of the third order
nonlinear susceptibility by a vibrational mode of a molecule. Coherent Anti-Stokes Raman Scattering (CARS) can
provide similar vibrational information as a spontaneous Raman spectrum. The technique has been shown to be orders
of magnitude more sensitive than spontaneous Raman, with video rate imaging demonstrated recently. In this work,
we investigate the potential use of broadband CARS spectroscopy and CARS imaging for biochemical analysis of
arterial tissue. Biochemical imaging data from broadband CARS is compared with spontaneous Raman
microspectroscopy. The broadband CARS system comprised of a single femtosecond-laser is presented in detail.
Issues related to data analysis, the advantages and current limitations of the CARS technique in biodiagnostics are
discussed.
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