脱氢表雄酮诱导CD4(上标 +)T胞系Jurkat细胞凋亡的初步探索

The aim of this study is to investigate effects of DHEA on the viability and apoptosis of Jurkat cells, in which the effect of DHEA on apoptosis in Jurkat cells was determined by DNA ladder, Hoechst33258, PT staining and Annexin-VFITC/PI double staining. RT-PCR method was used to analyze Fas and FasL mRNA expression and the Fas/FasL proteins were evaluated by flow cytometry. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence. It was demonstrated viability of Jurkat cells was down regulated, and numbers of Annexin V-positive and propidium iodide-negative cells were increased significantly after DHEA treatment. The sub-diploid peak of cells with fragmented DNA were also increased vs control. Agarose gel electrophoresis of DHEA treated Jurkat cells showed DNA characteristic fragmentation of apoptotic cells. JC-1 staining showed cells with green fluorescence increased significantly vs control. Fas and FasL mRNA and protein expression of Jurkat cells was also increased in DHEA-treated classes than that of controls. The results revealed that DHEA could induce apoptosis in Jurkat cells in vitro and the Fas/FasL pathway and mitochondrion membrane potential mutation play roles in these regulations.