Imaging Endogenous HClO in atherosclerosis using a novel fast-response Fluorescence Probe

A novel pyrene-based naked-eye colorimetric and fluorescent turn-on probe (S-ClO) possessing a donor-π-acceptor (D-π-A) archetype for the action of HClO detection in aqueous solution was designed and developed. The off-on signal sensing of HClO is ascribed to the high oxidative strength of HClO towards the double bond between the pyrene moiety and the pyrimidine moiety of S-ClO, resulting in oxygenation of the double bond to an aldehyde group. S-ClO shows low cytotoxicity，fast response time (5 s), high sensitivity, an extremely low detection limit (53 nM) towards HClO. Besides, imaging in living RAW 264.7 cells using S-ClO switch-on signal behaviour indicates its excellent capability in accurate and rapid detection of endogenous HClO. In particular, S-ClO was successfully applied in vivo by imaging Arterial Vessels inflammation nude mouse model. To further confirm the application value of S-ClO, it was successfully applied to detect HClO in human serum. Thus, S-ClO could serve as a potential tool for early diagnosing and monitoring inflammatory diseases.