Monitoring of 3D breast carcinoma cell culture using proton magnetic resonance imaging

We studied two human mammary carcinoma cell lines, MCF-7(NEO-4) HER-2 negative and MCF-7(HER-2) HER-2 positive, in three-dimensional culture. The aim of the study was to determine the glycosaminoglycan (GAG) concentration in breast cancer cell growth ex vivo. We found that GAG concentration produced by both breast cancer cell lines can be measured with high-field magnetic resonance imaging.