Research on Expression of Hematopoietic and Neural Markers in Human Placenta

The expression of neural and hematopoietic markers in placenta tissue and cultured human placenta derived adherent cells(hPDACs) and their correlation in embryonic development were investigated in this paper.The function of human placenta in hematopoiesis was demonstrated by immunocytological study in 7 and full-term months'placental slices.Positive reactions were observed in 7 month placenta using antibody against SCF,VEGF,BMP-4,FGF-1,CD34,CD133 and KDR.However,full-term months'placenta just reacted slightly with these antibodies.The staining for neural markers such as Nestin and MAP_2 showed positive reactions in 7 month and negative in full-term placenta.GFAP was showed negative in all two groups(placenta's) dying.The isolated adherent cells of placenta were positive for AKP,vitamin,SCF,VEGF,BMP-4, FGF-1,CD133,Nestin and MAP_2 markers,while they were negative for CD34,GFAP and MBP.They showed neural differentiation potential under appropriate conditions.The data suggest that placenta can express hematopoietic related factors to support hematopoiesis,and placental-derived cells have neural differentiation potential.