Determination of distribution of hepatitis B virus genotype in infected population in Chongqing region by real-time fluorescent genotyping and quantitative PCR

Objective To investigate the distribution character of hepatitis B virus(HBV) genotype in infected population in Chongqing region by real-time fluorescent genotyping and quantitative PCR as well as its clinical significance.Methods The HBV genotypes of 207 infected subjects were determined by real-time genotyping and quantitative PCR(GQ-PCR),and the results were verified by direct sequencing.Results Of the 207 samples,204 were successfully genotyped,while 3 were not.Of the 204 samples,127(61.35%) were judged as genotype B,29(14.01%) as genotype C,48(23.19%) as genotype B / C.The virus loads of samples of genotype C were significantly higher than those of genotype B and B / C(P 0.05).However,no significant difference was observed between the virus loads of samples of genotypes B and B / C(P 0.05).A total of 90 samples were randomly selected and genotyped by GQ-PCR and direct sequencing respectively,and the results were highly in agreement(Kappa = 0.834,P 0.05).Conclusion Genotype B is prominent in Chongqing region,secondary with genotype B / C.The mixed infection rate was higher than those reported previously.The HBV-DNA level of patients of infected with genotype C was higher than those with genotype B and B / C,which might be associated with more serious liver lesion caused by genotype C.