Penisilinle oluşturulan epilepside kolinerjik ve nitrerjik maddelerin diken amplitüdüne etkisi The effects of cholinergic and nitrergic substances on spike amplitude in penicillin induced epilepsy

Literaturde asetilkolinin (ACh) ve nitrik oksitin (NO) epilepsideki yeriyle ilgili calismalar vardir. Bununla birlikte herhangi bir epilepsi modelinde muskarinik kolinerjik sistem ile nitrerjik sistem arasindaki etkilesim bilinmemektedir. Sunulan calismanin amaci, penisilin modeli deneysel epilepside, nitrerjik sistem ile muskarinik kolinerjik sistemin etkilesimini arastirmaktir. Deneylerde agirliklari ortalama 220±35 gram olan 70 tane Wistar cinsi eriskin erkek sican kullanildi. Bu hayvanlar kontrol (200 IU/1μl penisilin), sodyum nitropurisid (SNP) (50 μg/5 μl), Nω-nitro L-arjinin metil ester (L-NAME) (100 μg/5 μl), ACh (250 μg/5 μl, i.c.), atropin (100 ng i.c.), atropin+SNP (100 ng atropin ve 10 dk sonra 50 μg SNP i.c.), atropin+L-NAME (100 ng atropin sulfat ve 100 μg/5 μl L-NAME i.c.), L-NAME+ACh (100 μg/5 μl L-NAME ve 10 dk sonra 250 μg/5 μl ACh i.c.), ACh+SNP (250 μg/5μl ACh ve 50 μg SNP i.c.) ve Atropin+ACh gruplarina ayrildi. Calismamizin sonuclarina gore intrakortikal penisilinden (200 IU/1 mikro litre) 2-5 dakika sonra ECoG’de epileptiform aktivite basladi. Asetilkolin ile SNP birlikte verildiginde, 10. dakikadan itibaren deneylerin sonuna kadar penisilinin olusturdugu epileptiform aktivite ve diken yukseklikleri, istatistik acidan cok onemli olcude baskilandi. Muskarinik kolinerjik ve nitrerjik sistemlerle ilgili diger uygulamalar ve diger maddeler diken yuksekliklerini istatistiksel acidan onemli sayilabilecek olcude etkilememistir. Sonuc olarak deney sartlarinda, asetilkolin tek basina uygulandiginda penisilinin olusturdugu epileptiform aktiviteyi siddetlendirmekte; fakat bir NO verici olan SNP ile birlikte verilince antikonvulzan etki artmakta ve diken yukseklikleri azalmaktadir. The effects of cholinergic and nitrergic substances on spike amplitude in penicillin induced epilepsy The role of acetylcholine (ACh) and nitric oxide (NO) in epilepsy is already known. There is no study on the interactions between cholinergic and nitrergic systems in an epilepsy model. The aim of this study was to investigate the interaction between muscarinic cholinergic system and nitrergic system in the experimental model of penicillin epilepsy. Seventy adult male Wistar rats weighing 220±35 g were used in the experiments. The experimental groups consist of control (200 UI/1 μl penicillin), SNP (50 μg/5 μl), N ω-nitro-L-arginine methyl ester (L-NAME)(100 μg/5 μl), ACh (250 μg/5 μl), atropin (100 ng,), atropin+SNP (100 ng atropine and 10 min later 50 μg SNP), atropin +L-NAME (100 ng atropine sulphate and 100 μg/5 μl L-NAME,), L-NAME+ACh (100 μg/5 μl L-NAME and 10 min later 250 μg/5 μl ACh ), ACh+SNP (250 μg/5 μl ACh and 50 μg SNP) and Atropin+ACh groups. According to the this study, epileptiform activity started within 2-5 min following the intracortical injection of penicillin (200 UI/1 μl). When ACh and SNP were administered together, penicillin induced epileptiform activity and spike amplitudes were significantly suppressed from the 10th min onwards. Amplitudes of the spikes induced by penicillin were significantly reduced in ACh+SNP group while the other compounds related to muscarinic cholinergic and nitrergic systems did not affect it. It can be concluded that acetylcholine increases the epileptiform activity induced by penicillin when administered alone while the anticonvulsant effect is increased when it is administered together with NO donor SNP. J. Exp. Clin. Med., 2012; 29:304-310