High performance thin layer chromatographic method for simultaneous estimation of paracetamol and valdecoxib in tablet dosage form

A new simple, sensitive and precise high performance thin layer chromatographic method has been developed for estimation of paracetamol and valdecoxib simultaneously from a combined dosage form. In this method pre coated silica gel 60 GF 254 TLC plate was used as stationary phase and the chromatogram was developed using chloroform: isopropyl alcohol: glacial acetic acid (9.5:1:0.2 v/v/v) as mobile phase. Paracetamol and valdecoxib showed Rf values 0.14±0.01 and 0.51±0.03, respectively. The plate was scanned and quantified at 250 nm using Camag TLC Scanner. The linear concentration was 2.5 to 12.5 µg/spot for paracetamol and 0.1 to 0.5 µg/spot for valdecoxib. The %RSD of intra day variation was 0.18±0.01 for paracetamol and 0.24±0.01 for valdecoxib. For inter day variation the %RSD was 0.03±0.005 and 0.1±0.02 for paracetamol and valdecoxib, respectively.