Establishment of analytical methods for HPHC list of mainstream cigarette smoke

Abstract: Harmful and potentially harmful constituents (HPHCs) are chemical compounds in tobacco smokethat cause harm to smokers and non-smokers. This study established and validated methods for the analysis ofHPHCs from mainstream cigarette smoke. The analyzed HPHCs were categorized into seven groups: aromaticamines, volatile organic compounds (VOCs), heavy metals, tobacco specific nitrosamines (TSNAs), benzo[a]pyrene(B[a]P), ammonia, and carbonyl compounds. The methods were validated by specificity, linearity, limit of detection(LOD), accuracy, precision, and recovery. These validated methods were then applied to the reference cigarettes(1R5F, 3R4F). The correlation coefficients (r 2 ) for the calibration curves of the seven groups were over 0.995. TheLODs showed values of 0.01-0.04 ng/cig cig for aromatic amines, 0.01-0.16 µg/cig for VOCs, 0.01-1.27 ng/cigfor heavy metals, 0.06-0.28 ng/cig for TSNAs, 0.04 ng/cig for benzo[a]pyrene, 0.08 µg/cig for ammonia, and 0.78-1.77 µg/cig for carbonyl compounds. The precisions obtained from the intra and inter-day batches were less than15%. The accuracy and the recovery range were less than 15% and 79.2-117.5%, respectively. The proposed methodscan therefore be applied for determining HPHCs in tobacco mainstream smoke. 요약: 본 연구를 통하여 담배 주류연에 포함되어 있는 HPHCs (Harmful and potentially harmfulconstituents) 중 7개 그룹의 분석법을 확립하였다. 이 7개 그룹에는 방향족 아민, 휘발성 유기화합물, 중금속, 담배특이니트로사민, 벤조[a]피렌, 암모니아, 카르보닐 그룹이 있다. 시험법 검증을 위하여 표준담배인 1R5F와 3R4F를 사용하여 분석법의 특이성, 직선성, 정성한계, 정량한계, 정확도, 정밀도 및 회수율을 확인하였다. 모든 상관계수(r