Intensification of surfactant synthesis of acinetobacter calcoaceticus IMV B-7241 on ethanol in presence of citrate and fumarate

The influence of the fumarate (C4-dicarboxylic acids precursors of the gluconeogenesis) and citrate (the regulator of lipids synthesizes), optimal pH of biosurfactant synthesis during Acinetobacter calcoaceticus IMV В-7241 cultivation on ethanol was studied. It was determined that the neutralization of the medium by the KOH solution in the process of the IMV B-7241 strain cultivation with the subsequent adding of the fumarate (0.01 %) and citrate (0.01 %) at the end of the exponential growth phase was accompanied by the increasing surfactant synthesis by 1.2-fold comparing to the same indexes without neutralization and by 3.5fold comparing to the cultivation of bacteria on ethanol without organic acids and pH regulation. The increasing of the surfactant synthesis in the presence of the fumarate ant citrate was caused by the 1.7–7-fold increased activity of the biosynthesis enzymes of glycolipids (phosphoenolpyruvate (PEP)-synthetase and thregalozophosphate-syntase) and aminolipids (NADP+dependent glutamatedehydrohenaze) as well as the simultaneous functioning of two anaplerotic ways (glyoxylate cycle and phosphoenolpyruvatecarboxylate reaction).