Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma

Abstract A high-performance liquid chromatographic method for the analysis of doxycycline in turkey plasma samples using demeclocycline hydrochloride as the internal standard was developed, optimized and validated, A one-step extraction procedure and an isocratic HPLC method with UV detection were used. No interferences with endogenous compounds or with the anticoagulant were observed, Linear calibration curves ( r 2 >0.99) were obtained in water and plasma between 0 and 600 μg ml −1 . Good recoveries for doxycycline (>66%) and demeclocycline (>72%) were seen both in water and in plasma, The coefficient of variation was −1 in plasma samples. A good stability of doxycycline was observed in water and in plasma samples after storage for six months at −20°C (recovery >91%).