Quantifying cell polarities in confocal images using 3D wall meshes
2019
Polarized transport of signaling molecules, such as the phytohormone auxin, is a core process for the
establishment of gradients involved in the patterning of multicellular plant tissues. Cell-to-cell polarity
of transport can result from the differential deposition of efflux carriers on the membranes at the interface
between cells, as for the pin-formed1 (PIN1) auxin transporter. Quantifying such sub-cellular information
in whole organ microscopy images constitutes a real challenge. In this work, we propose a method that
computes polarities at the level of cell-to-cell interfaces starting from standard resolution confocal
images and using a 3D geometric representation of cell walls. A robust estimation of spatial fluorescence
distribution around cell walls allows quantifying polarity with a fair level of confidence, and opens the
way for the automated analysis of polar transport at tissue scale.
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