Effect of Tian ma Gou teng Yin on serum Ca~(2+) and L-type Calcium Channels in the Vascular Smooth Muscle Cells in Spontaneously Hypertensive Rats(SHR)

Objective: To investigate the effect of Tian ma Gou teng Yin on blood pressure,the electricity physiology feature of L-type calcium channels in the vascular smooth muscle cells in SHR,to explain the mechanism of Tian Ma Gou Teng Yin to blood pressure.Methods: 12-week-old SHRs were assigned randomly into five groups.After 4-week-treatment,systolic blood pressure(SBP),and serum free calcium concentration were measured,the electricity physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique(PCT).Results: ①After 4-week-treatment,SBP was decreased in Tian ma Gou teng Yin group(P0.01);②No significant difference of Tian ma Gou teng Yin was found in the serum free calcium concentration(P0.05);③Tian ma Gou teng Yin can degradute the L-type calcium channels current of vascular smooth muscle cells.Conclusions: ①Both Tian ma Gou teng Yin and Nifedipine could repress early phase hypertension while no apparent statistical significance was found between them.The long-term antihypertensive effect of Tian ma Gou teng Yin is superior to Nifedipine;②Tian ma Gou teng Yin can block the L-type calcium channels current of vascular smooth muscle cells,which maybe one of the mechanisms of its antihypertensive function.