EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES

Abstract Seven Kinds Of Alkyl Alcohol (N-Butanol, N-Pentanol, N-Hexanol, N-Heptanol, N-Octanol, N-Nonanol, And N-Decanol) Were Chosen As The Cosolvent For Aliquat 336/Isooctane Reversed Micelles. By Performing Liquid-Liquid Extraction With Aliquat 336 Reversed Micelles As The Extractant, The Separation And Purification Of Two Enzymes (α-Amylase And Neutral Protease) From Bacillus Subtilis Were Investigated. Experiments Revealed That When N-Butanol Is Used As The Cosolvent, The Two Enzymes From Bacillus Subtilis Can Be Effectively Separated And Purified After A Full Forward And Backward Extraction Cycle, α-Amylase Was Separated In The Stripping Solution With About 85% Of Its Total Activity Recovered And Purified About 1.6-Fold. Neutral Protease Was Separated In The Raffinate With About 80% Of Its Total Activity Recovered And Purified About 3.5-Fold. For The Other Six Alcohols Used As The Cosolvent For Aliquat 336/Isooctane Reversed Micelles, Separation Was Not Achieved.