Bacillus CP-1 for lead-chromium pollution restoration and application of bacillus CP-1
2014
The invention relates to bacillus CP-1 for lead-chromium pollution restoration and an application of the bacillus CP-1 which can be used for restoring microorganisms polluted by lead and chromium. A preparation method for the bacillus CP-1 for lead-chromium pollution restoration comprises the following steps: preparing chromium pollution soil and sterile water into a suspension liquid, coating the suspension liquid onto an LB solid culture medium to culture, picking each single colony and marking out the single colony on a LB solid culture medium flat plate through a flat plate marking method, culturing over night in a 37-DEG C constant-temperature incubator; respectively inoculating the selected single colony onto a hexavalent chromium-containing LB solid culture medium flat plate and a lead-containing LB solid culture medium flat plate to culture, picking each single colony, marking out the single colony on the LB solid culture medium flat plate through the flat plate marking method and culturing over night; separating and purifying a bacterial strain, measuring the bacillus P-1 (Bacillus sp) through physiology and biochemistry and 16SrDNA, respectively naming the bacillus P-1 as bacillus with a preservation number of CGMCC No.9989; and performing domestication on bacillus P-1 (Bacillus sp) of a K2Cr2O7 LB liquid culture medium and a (CH3COO)2Pb LB liquid culture medium to obtain a seed solution, thereby preparing the bacillus CP-1 for lead-chromium pollution restoration. The bacillus CP-1 for lead-chromium pollution restoration disclosed by the invention can be used for effectively removing lead and hexavalent chromium, is simple to operate, economical and effective, and environment-friendly.
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