A Simple and Rapid Method for Isolation of High Quality Genomic Dna from the Biofuel Plant – Jatropha Curcas L.

A protocol for Jatropha curcas genomic DNA isolation based on a cetyl alkyltrimethyl-ammonium bromide (CTAB) method described for other woody species was optimized. The method involves mortar grinding of tissue, a modified CTAB extraction employing high salt concentration, polyvinyl pyrrolidone, successive isoamyl alcohol-chloroform extractions and an RNase treatment. High quality intact DNA (260/280 = 1.87) was isolated. The routine yield was approximately 40 (mue)g DNA per 100 mg of initial fresh plant material. The genomic DNA obtained by this method is suitable for use in random amplified-polymorphic DNA reactions. This extraction method allows molecular analysis of shoots from different accessions distributed throughout the country.