[Anti-tumor effect of adenovirus-mediated Bcl-XL shRNA in vitro].

Objective To investigate the anti-tumor effect of adenovirus-mediated Bcl-XL shRNA on colon cancer cells in vitro.Methods A recombinant Bcl-xl adenovirus was constructed,applified,and purified.The effect on mRNA expression of Bcl-XL was assessed by RT-PCR,and the effect on apoptosis-induction of colon cancer (Lovo cell line) in vitro was assessed by MTT assay and cell clonogenic assay.Results RT-PCR showed that Ad/Bcl-XL shRNA significantly down-regulated the mRNA expression of Bcl-XL in Lovo cells.Ad/Bcl-XL shRNA suppressed the proliferation of Lovo cells in a dose-dependent as well as a time-dependent manner compared with Ad/GFP(P＜0.05).Treatment with Ad/Bcl-XL shRNA dramatically suppressed the colony formation of Lovo cells in a dosedependent manner (P＜0.05).Ad/Bcl-XL shRNA showed no effect on normal human fibroblast.Conclusion Ad/Bcl-XL shRNA exhibits cytotoxic effect on Lovo cells and may have the potential value in the treatment of colon cancer. Key words: Colonic neoplasms; Adenovirus; Bcl-XL; Lovo cell line