Simultaneous estimation of amlodipine and losartan potassium in pharmaceutical dosage by reverse phase high performance liquid chromatography

A simple, fast and precise reverse phase high performance liquid chromatographic method developed for the simultaneous determination of Amlodipine and Losartan Potassium (LP) in its tablet form. A YMC-Pack C-18 ODS-A, 5pm, 120A column 15 cm x 4.6 mm i.d in isocratic mode, with mobile phase 50 mM KH 2 PO 4 , acetonitrile (60:40 v/v) pH adjusted to 3.00′0.1 with 5% o-phosphoric acid were used. The flow rate was 1.0 ml/min and effluent was monitered at 215 nm. The retention time of Amlodipine and LP were 4.19 min & 6.57 min respectively. Linearity for Amlodipine and LP were in the range of 0.010-0.050 mg/ ml and 0.100-0.500 mg/ml respectively. Amount found of Amlodipine and LP were 5.02 mg/tab and 50.06 mg/tab respectively. Percentage recoveries obtained for both the drugs were 99.08% and 99.51% for Amlodipine and LP respectively. The proposed method is accurate, precise, selective and rapid for the simultaneous estimation of Amlodipine and LP.