Quantitative Detection of Trace Malachite Green in Aquiculture Water Samples by Extractive Electrospray Ionization Mass Spectrometry

Exposure to malachite green (MG) may pose great health risks to humans, thus it is of prime importance to develop fast and robust methods to quantitatively screen the presence malachite green in environment. Herein application of extractive electrospray ionization mass spectrometry (EESI-MS) has been extended to the trace detection of MG within lake water and aquiculture water, due to the intensive use of MG as biocide in fisheries. This method has the advantage of obviating offline liquid-liquid extraction or tedious matrix separation prior to the measurement of malachite green in native aqueous medium. The experimental results indicate that the extrapolated detection limit for MG was ~3.8 ug L-1 (S/N=3) in lake water samples and ~ 0.5 ug L-1 in ultrapure water under optimized experimental conditions. The signal intensity of MG showed good linearity over the concentration range of 10–5000 ug L-1. Measurement of practical water samples fortified with MG at 0.01 and 0.1 mg L-1 gave a good validation of the established calibration curve. The average recoveries of malachite green in lake water and Carassius carassius fish farm effluent water were 114.9% (6.6% RSD) and 85.4% (9.2% RSD), respectively. Overall, the established EESI-MS/MS method has been demonstrated suitable for sensitive and rapid ( Subject Areas extractive electrospray ionization; rapid detection; malachite green; water; mass spectrometry Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Get PDF Cite Share Readers' Comments and Ratings (0) Your full nameYour affiliation information (e.g., name of your university/institution/organization)Your Email address (not made public)Importance: How significant is the paper to the field?Outstanding/highlight paperSignificant contributionIncremental contributionNo contributionI am not qualified to judgeSoundness of evidence/arguments presented:Conclusions well supportedMost conclusions supported (minor revision needed)Incomplete evidence (major revision needed)Hypothesis, unsupported conclusions, or proof-of-principleI am not qualified to judgePlease leave your comment to this article below.12ΣΩMathematical equations can be typed in either LaTeX formats \\[ ... \\] or $$ ... $$, or MathML format ... . Try the LaTeX or MathML example.Type equation: Preview: Copy equation into message below Close menuPlease click a symbol to insert it into the message box below:†‡‰♠♣♥♦‾←↑→↓™…–—i¢£¤¥¦§©a«¬­®¯°±²³´µ¶·¹o¼½¾?AAÂAAAAECEEEEIIIIÐNOOOOO×OUUUUÝÞsaaâaaaaeceeeeiiiiðnooooo÷ouuuuýþyΑαΒβΓγΔδEeΖζΗηΘθΙιΚκΛλΜμΝνΞξΟοΠπΡρΣσΤτΥυΦφΧχΨψΩω●• Close menuPlease enter the link here:Please enter a valid URLOptionally, you can enter text that should appear as linked text: Copy link into message below Close menuPlease enter or paste the URL to the image here (please only use links to jpg/jpeg, png and gif images):Please enter a valid URL Copy image into message below Close menu Type author name or keywords to filter the list of references in this group (you can add a new citation under Bibliography): No existing citations in Discussion GroupWikify editor is a simple editor for wiki-style mark-up. It was written by MDPI AG for Sciforum in 2014. The rendering of the mark-up is based on Wiky.php with some tweaks. Rendering of mathematical equations is done with MathJax. Please send us a message for support or for reporting bugs. Close menuPlease declare any conflict of interests that your comment might be involved, either financial or non-financial.I do not have any conflict of interest to comment on this article.I would like to declare conflict of interest.I accept Preprints User Comments Terms and Conditions Comments must follow the standards of professional discourse and should focus on the scientific content of the article. Insulting or offensive language, personal attacks and off-topic remarks will not be permitted. Comments must be written in English. Preprints reserves the right to remove comments without notice. Readers who post comments are obliged to declare any competing interests, financial or otherwise. Cancel Post Discuss and rate this article