Acipenser dabryanus spermatogonium culture solution and application thereof

The invention discloses an acipenser dabryanus spermatogonium culture solution and application thereof. According to the culture solution, a low-serum culture medium which does not have fetal calf serum, adopts various growth factors, nonessential amino acid, sodium pyruvate and other additives as substitutes and is assisted by a small amount of male acipenser dabryanus serum is provided so as to guarantee mass reproduction of spermatogonium and low reproduction rate of a spermatic cell; and the spermatic cell 13d which is cultured to the 13rd day are doped with 25mu M BrdU and is marked as a newly reproduced cell. 24h immunohistochemical experiments indicate that the ratio of germ cell marked cells in newly reproduced cells after BrdU is doped for 24 hours is 85.78 percent. According to the culture solution, the current problem that the acipenser dabryanus spermatogonium passage cannot be massively cultured can be solved, and an effective cell platform is provided to acipenser dabryanus reproductive development researches.