LC and SPE Determination of Chlorophacinone in Corn Grains, Soil and Rat Plasma

A liquid chromatographic method was used for the analysis of chlorophacinone in corn, soil and rat plasma. Sample preparation differed from corn, soil and serum. Simple liquid extraction procedures were applied to soil and corn; solid phase extraction using Oasis HLB cartridges and easy protocol was used for plasma. Liquid chromatography was on X-terra C18 column. The mobile phase used did not contain ion-pairing reagent, it was constituted with methanol/ammonium bicarbonate (0.01 M, pH = 7), 70/30, v/v at 1 mL min−1. UV detection was by using photodiode detector operating at 282 nm. Complete validation of the proposed procedure for corn, soil and serum was made.