Maternal protein restriction depresses the duodenal expression of iron transporters and serum iron level in male weaning piglets

To investigate the effects of maternal dietary protein restriction on offspring Fe metabolism, twenty-four second-parity Landrace×Yorkshire sows were randomly allocated to standard-protein (SP) and low-protein (LP) groups. The SP sows were fed diets containing 15 and 18 % crude protein throughout pregnancy and lactation, respectively, whereas the LP sows were subjected to 50 % dietary protein restriction. Offspring birth weight was not affected, but the body weight at weaning ( P =0·06) and average daily gain ( P =0·01) of the female piglets were significantly decreased. Serum Fe level in the LP piglets was markedly decreased at weaning, especially in males ( P =0·03). Serum ferritin level ( P =0·08) tended to be lower, yet serum transferrin was greatly higher ( P =0·01) in male weaning piglets of the LP group. Duodenal expression of the divalent metal transporter 1 (DMT1) and ferroportin (FPN) was surprisingly reduced ( P P =0·09), lower hepatic expression of transferrin ( P P