HPLC analysis of Sangtangyin oral solution under different process conditions.

A method for monitoring Sangtangyin oral solution under different process conditions by HPLC was established and the influence of process variables on fingerprint peaks of Sangtangyin oral solution was studied. Samples was analyzed by HPLC with Eclipse XDB C18 as chromatogram column and methanol –1% acetic acid(gradient elution)as a mobile phase at 254nm.The flow rate and column temperature were 1mL·min-1 and 38℃, respectively. 26 Kinds of common peaks were found in the two samples at different process conditions. The adopted process conditions showed a little influence on characteristic peaks of the samples. The quality of sample 2# was a little better than that of sample 1#. The method was reliable, accurate and provided a reference for the further study of Sangtangyin oral solution.