Three-dimensional analysis of the intermediate filament network using SEM-tomography

Pancreatic cancer is characterized by its early infiltration of adjacent tissue. There is a correlation between the keratin filament network structure and the migration ability of cells [1]. In earlier papers we quantitatively investigated two-dimensional images of the three-dimensional keratin network [2]. It is the goal of our ongoing research to expand these studies to three-dimensional datasets. We investigated, therefore, intermediate filaments from detergent-extracted cells by SEM-tomography.