Hyper‐spectral Raman imaging correlating chemical substitution and crystallinity in biogenic hydroxyapatite: Dentin and enamel in normal and hypoplastic human teeth

Correlative hyperspectral Raman imaging with high spatial and spectral resolution, precision with peak position and linewidth analysis, and from the μm to mm scale, provides new insight into structural characteristics in dentin and enamel. We determine variations in degree of crystallinity, both locally across the dentin–enamel junction, and with distinct long‐range spatial variations. We identify a correlation of spectral peak position and linewidth as a measure of crystal lattice disorder across tubules, dentin, dentin–enamel junction, and enamel.