A kind of fermentation technology of Pichia anomala expression recombiant protein

The invention discloses the fermentation technology of a kind of Pichia anomala expression recombiant protein, using pichia pastoris phaff Pichia pastoris is strain, first carry out first order seed cultivation, to bacterium dense when being 20 ± 2g/L switching secondary seed cultivate, secondary seed is cultivated to bacterium and dense is forwarded to glycerol cultivation stage when being 120 ± 10g/L, in glycerol cultivation stage, the addition of glycerol is 60～70g/L, when dissolved oxygen rapidly rises to reach metastable state, enter methanol induction phase, after induction 120 ± 8h, fermentation ends.The present invention reduces the glycerol feeding stage in original technique, glycerol runs out of and i.e. enters next stage, it is no longer necessary to prepares stream and adds with sterile glycerol, has simplified glycerol sterilization tank, decreased the energy, the loss of resource and the waste of glycerol；Simultaneously without monitoring cell concentration again, reducing probability of makeing mistakes, reduce the fermentation failure caused because of technology mistake, be more suitable for large-scale industrial production, the industrialized production for recombiant protein offers convenience and great economic worth.