Hyperbranched polyglycerol β-cyclodextrin as magnetic platform for optimization of doxorubicin cytotoxic effects on Saos-2 bone cancerous cell line

Abstract In recent years, cancer has become the most important human health problems in the world. To overcome these problems, chemotherapy as a treatment approach is widely used in cancer treatment. However, single chemotherapy have a toxic side effects without completely effectiveness. This study has focused on the mitigating of doxorubicin side effects by designing pH-sensitive magnetic hyperbranched β-cyclodextrin as a nano-sized drug carrier. The accuracy of samples preparation has been confirmed by several characterizations including FTIR, NMR, SEM, EDX, TEM, XRD, DLS and Zeta Potential tests. The hemolysis assay results have shown that synthesized nanocarrier is completely biocompatible and could be used in blood contacting applications. In this case, nanoparticles have a better release in acidic pH value. In-vitro studies including MTT assay uptake assay, DAPI staining and apoptosis assay by flow-cytometry have been carried out on Saos-2 cell line. It is concluded that a synthesized doxorubicin-loaded nanocarrier has more cytotoxicity than free doxorubicin after incubation time, showing its high potentiality for target doxorubicin delivery to Saos-2 cell line.