INHIBITIONS OF SALIDROSIDE ON THE Aβ_(25-35)-INDUCED APOPTOSIS BY FRET IN LIVING PC12 CELLS

To investigate the inhibitions of salidroside on the apoptosis induced by Aβ_(25-35) in PC12 cells,cell survival was analyzed with cell counting Kit-8(CCK-8),cell morphology was observed by optical microscope,nuclear condensation was shown by Hoechst staining,the dynamics of caspase-3 and caspase-8 activation in single living cell were monitored by fluorescence resonance energy transfer(FRET) respectively.The results showed that salidroside elevated the cell survival by inhibiting the Aβ_(25-35)-induced apoptosis in a dose-dependent manner,and that salidroside greatly inhibited the caspase-3 activation.Furthermore,the apoptosis induced by Aβ_(25-35) was independent of the caspase-8 activation.In conclusion,these data indicate that salidroside can protect the PC12 cells from Aβ_(25-35)-induced apoptosis by inhibiting the activation of caspase-3.