Studies on Fingerprint Chromatograms of Anti-virus Component of Compound lndigowoad Root Granule by HPLC-UV and LC-ESI-MS(n)

The fingerprint chromatograms of the extracts from Compound Indigowoad Root Granule was established by HPLC-UV. Nineteen common peaks were found in fingerprint chromatograms of the extracts from Compound Indigowoad Root Granule and good similarities with correlation coefficients over 93% were found between the fingerprint chromatograms of the extracts from different sources. The main common peaks were identificated by HPLC-MS(n). Pharmacodynamic studies have shown that the extract from Compound Indigowoad Root Granule had anti-viral activity. This method with desirable accuracy, stability and repeatability can be used for the quality evaluation of Compound Indigowoad Root Granule.