Use of thiobabituric acid for inhibiting generation of melanin and biological pesticide

The invention discloses the use of thiobabituric acid for inhibiting generation of melanin and a biological pesticide. In the invention, the activity of tyrosinase is measured by spectrophotometry, the inhibiting factor Km of the tyrosinase is measured by using L-DOPA as a substrate and by a Lineweaver-Burk method, the activity of the tyrosinase is measured by reaction of typically 1 milliliter of reaction substrate system and 10 milliliters of enzyme solution, and in the research, the activity of the tyrosinase is measured by using a PerkinElmerLambdaBio ultraviolet and visible spectrophotometer to detect an absorption value, namely the value of v is indicated by the change per mine of absorption value at a 492-nanometer position. In the use of the thiobabituric acid as the tyrosinase for inhibiting generation of melanin, a fluorescence emission spectrum is detected by using a cuvette with a 1-centimeter optical path for a JascoFP750 fluorescence spectrometer.