Exploring the differences between mouse mAβ1–42 and human hAβ1–42 for Alzheimer's disease related properties and neuronal cytotoxicity

The differences between mouse mAβ1–42 and human hAβ1–42, explored using CD and fluorescence spectroscopy, transmission electron microscopy, ROS fluorescent assay, and neuronal cell viability, revealed that mAβ1–42 as a three-site mutant (R5G, Y10F and H13R) of hAβ1–42 altered the metal (copper and zinc) binding sites, reduced the proneness to form β-sheet structures and aggregated fibrils, alleviated the generation of ROS, and decreased the cytotoxicity, in contrast to hAβ1–42.