Conditionally reprogrammed co-culturing system in human parathyroid cell culture

Objective To in vitro culture human parathyroid primary cells using the conditionally reprogrammed co-culture (CRC) system for establishing a parathyroid chief cell line which can grow and transfer stably in vitro. Methods The irradiated 3T3-J2 cell line was combined with the Rho kinase inhibitor (Y-27632, 5×10-6 mol/L) and type I collagen to construct the CRC system. Human parathyroid primary cells were co-cultured in the CRC system. The growth activity and pathological features of human parathyroid cells were evaluated by methodology and parathyroid hormone concentration detected by chemiluminescent immunoassay. Results In CRC system, the co-culture time of parathyroid chief cell line was 50-55 days, with a significant culturing time window for 30-35 days. Total culture generation was 3. Parathyroid hormone was detected in the medium and fluctuated according to calcium ion concentration. Conclusion A relatively stable, physiologically active parathyroid chief cell line could be built successfully in the CRC system. Key words: Parathyroid gland; Culture in vitro; Conditionally reprogrammed cells co-culture