AMELIORATIVE EFFECT OF THREE MEDICINAL PLANTS (P. FRATERNUS, TERMINELIA A., AND MORINGA OLEIFERA) ON ARSENIC TRIOXIDE INDUCED ALTERATION OF LIPID PEROXIDATION AND PROTEIN CONTENTS IN CHICKEN LIVER HOMOGENATE: AN IN VITRO STUDY

The ameliorative effect of the aqueous extract of three medicinal plants P. fraternus, Terminalia a., and Moringa oleifera (PF, TA, and MO) on arsenic trioxide (As2O3) induced alteration in lipid peroxidation (LPO) and protein contents was studied in vitro. Liver from healthy chicken (Gallus domesticus) weighing 1.2 to 1.5 kg was bought to laboratory in frozen condition from local slaughter house and used for study. When 0.2 mL of liver homogenate was treated with 1-5 µg/mL of As2O3, it caused significant alterations in LPO and total protein content of chicken liver. The maximum alteration was observed at 3 mg/mL concentration of As2O3. Addition of each plant extract to liver homogenate did not caused significant alteration in LPO and protein con- tents. However, concurrent addition of As2O3 and plant extract (25-100 µg/mL) caused significant ameliorative effect. Ameliorative effect of each plant extract was studied separately. The maximum amelioration of PF, MO, and TA was observed at 50 mg/mL, 100 µg/mL, and 100 µg/mL respectively. Thus it is concluded that aque- ous extract of PF was observed to have better ameliorative effect.