[Protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis].

Objective To investigate the protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis. Methods By PCR technique, the complete open-reading frame sequences of Rv1246c and Rv1247c gene were amplified from the M.tuberculosis H37Rv genomic DNA as template. The PCR-amplified cDNAs of Rv1247c and Rv1246c gene were subcloned into pGBKT7 and pGADT7-Rec vector respectively for constructing recombinant plasmids pGBKT7-Rv1247c and pGADT7-Rv1246c. After verified by restriction endonuclease digestion and DNA sequence determination, the recombinant vectors were used to transform the yeast cell AH109 by lithium acetate method. Results The yeast cells co-transformed with pGBKT7-Rv1247c and pGADT7-Rv1246c grew on SD/-Ade/-His/-Leu/-Trp plates, and the β-galactosidase activity assays showed the positive signal. Conclusion The hypothetical protein Rv1246c and Rv1247c could interact with each other in yeast cells.