The correlation intranscriptional expression with promoter hypermethylation of AKAP12 gene in bladdertransitional cell carcinoma

：Objective To investigatetranscriptional expression and promoter CpG methylation status of A-kinase anchoringprotein 12 (AKAP12) gene and analyze their correlation with clinical pathological stage inbladder transitional cell carcinoma. Methods AKAP12 mRNA expression level and promoter CpGmetbylation status was measured by fluorescent quantitative RT-PCR (FQ-RT-PCR) andmethylation specific PCR (MSP) in 30 bladder transitional cell carcinoma and adjacentnormal tissues. The products of PCR were cloned and bisulfite sequenced. Results DecreasedAKAP12 mRNA expression was demonstrated in 22 carcinomas (73. 3% ) and was significantlyassociated with turnout grade (P =0. 02).The frequency of promoter methylation of AKAP12gene was 53. 3 % (16/30) and correlated with the tumor stage(r =0.52,Pn =0.03)and grade(r=0.61,Pn =0.01). Conclusion Aberrant promoter methylation of AKAP12 can result in the lossof gene expression and may association with bladder transitional cell carcinoma.