Glutathione disulfide-sensitive Janus nanomachine controlled by an enzymatic AND logic gate for smart delivery

2021 
This work describes the assembly of a novel enzyme-controlled nanomachine operated through an AND Boolean logic gate for on-command delivery. The nanodevice was constructed on Au-mesoporous silica Janus nanoparticles capped with a thiol-sensitive gate-like molecular ensemble at the mesoporous face and functionalized with glutathione reductase on the gold face. This autonomous nanomachine employed NADPH and glutathione disulfide as input chemical signals, leading enzymatic production of reduced glutathione that causes the disruption of the gating mechanism at the mesoporous face and the consequent payload release as output signal. The nanodevice was successfully used for the autonomous release of doxorubicin in HeLa cancer cells and RAW 264.7 macrophage cells.
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