Perfil de resistência a antimicrobianos de Staphylococcus spp. isolados de frangos de corte e poedeiras comerciais no

ABSTRACT.- Barros M.R., Costa M.M., Franca C.A., Saukas T.N., Silva L.B.G., Silva V.A.S., Caval-cante R.V. & Mota R.A. 2011. [ Resistance of Staphylococcus spp., to antimicrobials isolatedfrom broilers and commercial layers in the State of Pernambuco, Brazil. ] Perfil de resis-tencia a antimicrobianos de Staphylococcus spp., isolados de frangos de corte e poedeiras co-merciais no Estado de Pernambuco. Pesquisa Veterinaria Brasileira 31(8):672-676. Departa-mento de Medicina Veterinaria, Universidade Federal Rural de Pernambuco, Av. Dom Manuelde Medeiros s/n, Recife, PE 52171900, Brazil. E-mail: rinaldo.mota@hotmail.comThis study had the objective of researching Staphylococcus spp. on healthy broilers andcommercial broilers and layers, with clinical respiratory signs. Swabs were taken from theinfraorbital sinus of 55 healthy broilers, 35 with respiratory signs and 30 commercial layersalso with respiratory signs. Each sample was composed of a pool of five birds, totaling 24 collectedsamples from 24 commercial flocks. The bacteriological exam was used for the isolation, with alater evaluation of the morphological, tintorial and biochemical characteristics to determinethe species. The production of hemolysis, formation of a biofilm in Congo Red Agar, detection ofthe gene mecA by the PCR and susceptibility to 13 antimicrobial drugs, was verified. From the24 processed samples, 16 Staphylococcus spp. were obtained isolates, five samples werecoagulasis-positive (SCP) and 11 coagulasis-negative (SCN). As to hemolysis and the formationof biofilm tests three samples presented themselves to be hemolytic and six were positive,respectively. In the PCR evaluation for the detection of the mecA gene, all isolates showed negativeresults. It was observed that 15 E coli isolates were resistant to five or more antibiotics, and thatthe associated drugs presented better sensibility. The resistance to antimicrobials and biofilmproductive strains can interfere in the therapeutic response of birds that present clinical signs.