Relatedness of Escherichia coli colonizing women longitudinally.

Purpose: The longitudinal colonization patterns by Escherichia coli of the vaginal introitus and urinary tract were investigated. Materials and Methods: Cultures of the vaginal introitus and midstream urine were collected once a week for 12 consecutive weeks from five women with (patients) and five without (controls) a history of urinary tract infection (UTI). Results: A total of 63 E. coli isolates was obtained from the 10 women, 26 from controls and 37 from patients. The bacterial counts of E. coli present in control individuals were uniformly low, ≤ 200 E. coli/mL. The numbers in patients were higher and more variable, reaching > 105/mL in urine and vaginal specimens. In 16 instances, E. coli was present in the urine and the vaginal introitus concurrently (matched isolates). Random amplified polymorphic DNA (RAPD) fingerprinting was used to characterize all matched E. coli isolates. Concurrent vaginal and urinary tract colonization was more common in the patient population, and usually, the same E. col...