Crioconservación de yemas de microtubérculos de papa Solanum tuberosum ssp. andigena mediante desecado de tejidos

At the Corporacion de Investigacion Agropecuaria Corpoica, C.I. Tibaitata, the cryoconservation of microtubers buds was evaluated in two potato accessions of Solanum tuberosum ssp. andigena, C.C.C. 4318 (carriza) and C.C.C. 4981 (guata negra), from the Colombian Central Collection by using the dried bud methodology. This methodology had two steps, the preliminary one or adjustment and the main one. In the main step, the tissue drying was made with sterile air flow for 30 minutes, cryoconservation in liquid nitrogen at -196°C during 1 day, water bath thawing at 37°C for 3 minutes and recuperation culture medium (100% MS + 8% sucrose + 2 g/L activated charcoal) during 7 days in dark, then the cultured medium was changed (100% MS + 0.04 mg/L kinetin + 0.1mg/L gibberellic acid). A completely randomized experimental design with 2 accessions, 4 treatments, 10 replications and 10 buds, was used. The survival was evaluated at 7, 14 and 30 days. At the same time, the tetrazolium test on seeds was adapted at 0.5% a 25°C for 24 hours, and evaluated after 14 and 30 days. In this bioassay, the survival evaluation of the buds was difficult possibly due to the effect of culture medium and environmental factors. The final result was loss of survival at 30 days, probably because the recovery conditions were not suitable.