Culturing vessel and automatic culturing device

This culturing vessel is provided with: a first vessel; a plurality of second vessels accommodated and held within the first vessel; a vessel cover member that seals the first vessel and each of the second vessels; and connection ports that connect the first vessel and each of the second vessels to a flow path circuit that supplies cells and medium to the culturing vessel. The first vessel and the second vessels are each vessels for accommodating medium and cells or just medium. For the connection ports, the first vessel is connected with a second supply port for supplying medium and supplying and discharging a gas and a second discharge port for discharging medium, and each of the second vessels is connected with a first supply port for supplying medium and supplying and discharging a gas and a first discharge port for discharging the medium. The first supply port and the second supply port function as a flow path in which the medium always flows in one direction with respect to the culturing vessel.