Preliminary study on serum pharmacochemistry of petroleum ether fraction in ethanol extract from Xiaoyao Powder based on UPLC-PDA

Objective To study the adscription and identification of transitional constituents in serum of rats after ig administration of petroleum ether fraction in ethanol extract from Xiaoyao Powder(XYP-A).Methods A UPLC-PDA method was established to identify the active components in serum of rats after ig administration of XYP-A and the single preparations of Bupleuri Radix(BR)Angelicae Sinensis Radix(ASR),and Atractylodis Macrocephalae Rhizoma(AMR).The transitional constituents were analyzed by comparing the fingerprints of the serum samples in formula,the single preparations and reference substances,referring literature,retention time,and UV scan spectra.Results Twenty components including 12 original components from XYP-A and eight metabolites were detected in serum of rats after ig administration of XYP-A.The original components consisted nine derived from BR,two from ASR,one from AMR,and the structures of ligustilide,atractylenolide II,2,8,10-pentadecatriene-4,6-diyne-1-ol(CH-1),and bupleurynol(CH-2) were identified.Conclusion The method is successfully applied for the serum pharmacochemistry study in rats.Twenty transitional constituents are absorbed into serum of rats and their metabolites may be the effective constituents of XYP-A acting directly to the body,which could lay the foundation for the serum pharmacodynamic study.